论文部分内容阅读
目的检测食管癌和癌旁正常组织Ras相关区域家族1A(RASSF1A)基因启动子区甲基化变化。方法采用甲基化特异性聚合酶链反应(MSP)检测79例食管鳞癌患者癌组织及对应的20例癌旁正常组织RASSF1A基因启动子区甲基化,并观察食管癌组织RASSF1A基因启动子区甲基化与临床病理变化的关系。结果79例食管鳞癌组织RASSF1A基因启动子区甲基化阳性率(67%,53/79)明显高于癌旁正常组织(15%,3/20)。食管癌组织中不同年龄、分化程度的RASSF1A基因启动子区甲基化阳性率差异有统计学意义(P<0.05),而淋巴结转移与否、肿瘤分期间甲基化阳性率差异无统计学意义。20例同一个体癌组织甲基化阳性率明显高于癌旁正常组织(50%(10/20)vs15%(3/20))(P<0.05),仅3例同时出现甲基化,一致率为15%。结论RASSF1A基因启动子区甲基化是食管鳞癌频发的分子事件。食管鳞癌组织RASSF1A基因甲基化明显高于癌旁正常组织,并且和年龄、分化程度有关。
Objective To detect the promoter methylation of family RASSF1A gene in esophageal cancer and adjacent normal tissues. Methods Methylation-specific polymerase chain reaction (MSP) was used to detect the promoter region methylation of RASSF1A gene in 79 cases of esophageal squamous cell carcinoma and 20 cases of corresponding adjacent normal tissues. The expression of RASSF1A gene promoter Relationship between methylation and clinicopathological changes in. Results The positive rate of methylation of RASSF1A gene in 79 cases of esophageal squamous cell carcinoma (67%, 53/79) was significantly higher than that in adjacent normal tissues (15%, 3/20). The positive rate of methylation of RASSF1A gene in esophageal cancer tissues with different age and differentiation was significantly different (P <0.05), but there was no significant difference in the positive rate of methylation between lymph node metastasis and lymph node metastasis . The positive rate of methylation in 20 cases of the same cancer tissue was significantly higher than that in the adjacent normal tissues (50% (10/20) vs 15% (3/20)) (P <0.05), and only 3 cases had methylation at the same time The rate is 15%. Conclusion The promoter methylation of RASSF1A gene is a frequent molecular event in esophageal squamous cell carcinoma. The methylation of RASSF1A gene in esophageal squamous cell carcinoma tissues was significantly higher than that in adjacent normal tissues and was related to the age and differentiation degree.