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以金花茶浓缩液为试验材料,探讨了金花茶皂甙的初步分离纯化。金花茶浓缩液经过乙醇沉淀、乙醚脱脂处理后得到粗皂甙溶液。通过比较XAD1600、XAD16、DM130大孔吸附树脂、聚酰胺树脂等极性不同的树脂对皂甙的吸附及解吸效果,选择了XAD16大孔树脂作为粗皂甙的分离树脂。试验结果显示,粗皂甙通过XAD16大孔吸附树脂层析柱分离纯化后,在水,10%、20%和30%的异丙醇洗脱液处分别得到WS、IS1、IS2和IS3等4个皂甙组分。高效液相分析结果显示,相对于粗皂甙,各皂甙组分的吸收峰数量明显减少,表明XAD16大孔吸附树脂层析柱可以有效将金花茶粗皂甙分离出极性不同的皂甙组分。
Taking the golden flower tea concentrate as the experimental material, the preliminary isolation and purification of Ginsenoside was discussed. Camellia sinensis concentrate after ethanol precipitation, degreasing ether obtained crude saponin solution. XAD16 macroporous resin was chosen as the separation resin for crude saponin by comparing the adsorption and desorption effects of saponins with different polarities such as XAD1600, XAD16, DM130 macroporous adsorption resin and polyamide resin. The results showed that the crude saponins were separated and purified by XAD16 macroporous resin column, and then 4, such as WS, IS1, IS2 and IS3 were obtained respectively in water, 10%, 20% and 30% Saponin components. The results of HPLC showed that the number of absorption peaks of each saponin component decreased significantly compared with that of saponins, indicating that XAD16 macroporous resin column can effectively separate saponin components with different polarity.