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目的:观察转人类胰岛素样生长因子-Ⅰ(HumanInsulin -likeGrowthFactor-Ⅰ,hIGF -Ⅰ)基因成肌细胞回输小鼠骨骼肌钝挫伤局部后,对小鼠骨骼肌愈合过程中Ⅱb型肌球蛋白重链(MyosinHeavyChain -Ⅱb ,MHC -Ⅱb)及波形蛋白(Vimentin)mRNA表达的影响。方法:将90只7～12周雄性C3H小鼠右下肢腓肠肌中段内侧实施急性钝挫伤后,随机分为自然愈合组、单纯成肌细胞注射组和转基因成肌细胞注射组。挫伤后第3天,对单纯成肌细胞注射组、转基因成肌细胞注射组损伤局部分别注射等量单纯成肌细胞和转基因成肌细胞,并于损伤后第1、5、10、2 0、30天取材。检测不同时间点MHC -Ⅱb和VimentinmRNA表达水平,观察比较各组骨骼肌修复情况。结果:(1)在急性骨骼肌钝挫伤修复期,3组小鼠骨骼肌MHC -ⅡbmRNA均明显升高,第2 0天达到峰值。转基因成肌细胞注射组MHC -ⅡbmRNA表达水平最高,其次为单纯成肌细胞注射组。(2 )急性骨骼肌钝挫伤修复过程中,转基因成肌细胞注射组VimentinmRNA的表达水平始终在3组中表达最低。结论:急性骨骼肌钝挫伤修复过程中,注射转基因成肌细胞能提高损伤后MHC -IIbmR NA表达水平,促进肌纤维的增生,加速骨骼肌愈合进程。同时,损伤局部注射转hIGF -Ⅰ基因的成肌细胞后,使得损伤局部VimentinmRNA表达低于另两组,一
OBJECTIVE: To observe the effect of transfection of myofibroblasts derived from Human Insulin-like Growth Factor-I (hIGF-Ⅰ) gene on the contusion of skeletal muscle in mice, (Myosin Heavy Chain-IIb, MHC-IIb) and Vimentin mRNA expression. Methods: Ninety male Sprague-Dawley rats were randomly divided into three groups: natural healing group, simple myoblast injection group and transgenic myoblast injection group. On the third day after contusion, rats in the simple myoblasts injection group and the transgenic myoblasts injection group were injected with equal numbers of pure myoblasts and transgenic myoblasts separately, and the rats were sacrificed on day 1, 5, 10, 20, 30 days drawn. The expression of MHC-IIb and Vimentin mRNA at different time points were detected, and the repair of skeletal muscle in each group was observed and compared. Results: (1) MHC-Ⅱb mRNA in skeletal muscle of three groups of mice were significantly increased in the acute skeletal muscle contusion repair period and peaked on the 20th day. The expression level of MHC-Ⅱb mRNA in transgenic myoblasts was the highest, followed by that of simple myoblasts. (2) In the process of acute skeletal muscle contusion repair, the expression level of Vimentin mRNA in transgenic myoblasts was always the lowest among the three groups. CONCLUSION: Transgenic myoblasts can improve the expression of MHC-IIbmR NAA, promote the proliferation of myofibers and accelerate the process of skeletal muscle healing in acute skeletal muscle contusion repair. At the same time, after local injection of hIGF-I gene into myoblasts, the expression of Vimentin mRNA in local injury was lower than that of the other two groups