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为了培育高产优质苜蓿新品种,利用N+束介导方法将玉米DNA导入苜蓿种子,在当代获得了多种变异植株。采用AFLP分子标记技术,对株高、茎粗、分蘖数差异显著的5种变异植株(3-8、4-12、4-13、4-25、4-26)的基因组DNA进行多态性分析,结果表明,苜蓿变异植株与对照苜蓿间的AFLP扩增图谱相比,分别检测到16、14、12、20、13条差异带,差异率分别为6.3%、5.1%、4.4%、7.1%、4.7%,变异株与对照苜蓿基因组DNA多态性存在明显差异;变异株4-25中检测到与阳性对照玉米一致的2条DNA目的带,为离子束介导玉米DNA转化苜蓿提供了分子证据。
In order to cultivate high yield and high quality new alfalfa varieties, the introduction of maize DNA into alfalfa seeds by N + bundle mediated method has obtained many kinds of mutant plants in the contemporary era. Using AFLP molecular marker technology, the genomic DNA of five kinds of mutant plants (3-8,4-12,4-13,4-25,4-26) with significant differences in plant height, stem diameter and tillering number were analyzed for polymorphism The results showed that 16, 14, 12, 20 and 13 differential bands were detected in AFLP amplified bands of alfalfa and control alfalfa with the difference rates of 6.3%, 5.1%, 4.4%, 7.1 % And 4.7%, respectively. There were significant differences in the genomic DNA polymorphism between the mutant and the control alfalfa genomic DNA. The two DNA bands which were consistent with the positive control maize were detected in the mutant 4-25, which provided ion beam-mediated maize DNA transformation of alfalfa Molecular evidence.