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本文以2-氯-4,6-二氨-1,3,5-三嗪为虚拟模板分子,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,在纳米硅球表面合成了特异性吸附三聚氰胺的表面分子印迹聚合物(MIPs)。采用红外光谱、电镜等手段对印迹聚合物进行了表征;用静态吸附曲线和Scatchard方程评价了印迹聚合物对三聚氰胺的吸附性能。将合成的表面印迹聚合物作为填料制备分子印迹固相萃取(MIP-SPE)柱,优化了固相萃取条件,与液相色谱串联质谱联用建立了牛奶样品中三聚氰胺的MIP-SPE-HPLC/MS/MS分析方法。实验结果表明,以2-氯-4,6-二氨-1,3,5-三嗪为虚拟模板制备的MIPs能够特异性吸附三聚氰胺,其MIP-SPE柱可以富集净化牛奶样品中的三聚氰胺,添加回收率为80.9%~86.5%,相对标准偏差小于6.4%,定量限(10S/N)为0.023 mg/L。
In this paper, 2-chloro-4,6-diamino-1,3,5-triazine as a virtual template molecule, methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a crosslinking agent, Surface-imprinted polymers (MIPs) that specifically adsorb melamine were synthesized on the surface of the sphere. The imprinted polymers were characterized by means of infrared spectroscopy and electron microscopy. The adsorption properties of imprinted polymers on melamine were evaluated by static adsorption curve and Scatchard equation. The synthetic surface-imprinted polymer was used as filler to prepare MIP-SPE column. The conditions of solid-phase extraction (SPE) were optimized. The MIP-SPE-HPLC / MS / MS analysis method. The experimental results showed that MIPs prepared with 2-chloro-4,6-diamino-1,3,5-triazine as a virtual template could specifically adsorb melamine and its MIP-SPE column could enrich the melamine , The recovery rate was 80.9% ~ 86.5%, the relative standard deviation was less than 6.4%, and the limit of quantification (10S / N) was 0.023 mg / L.