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目的建立体位性低血压大鼠模型并进行初步应用。方法从倾斜角度(30°,45°,75°,90°)和倾斜时间(1,2 min)两方面优化建立大鼠低血压模型的条件,再用哌唑嗪(1.1~220μg·kg?1)和萘哌地尔(30~3 000μg·kg?1)多剂量给药,探讨模型有效性和可靠性。结果随着倾斜角度的增加,大鼠的血压和心率变化幅度加大,体位持续时间与血压、心率的变化没有明显的相关性。哌唑嗪剂量依赖性强化了体位性低血压,对血压和心率都表现出明显的抑制作用;萘哌地尔仅在3 000μg·kg?1剂量表现出对血压和心率的抑制效应。结论 45°头朝上位倾斜、持续时间60 s是建立体位性低血压大鼠模型的最适条件,哌唑嗪和萘哌地尔检验的结果与文献报道一致,证明了本模型有效、可靠。
Objective To establish a model of orthostatic hypotension and to conduct preliminary application. Methods The hypothesis of hypotension model was optimized from the angle of inclination (30 °, 45 °, 75 °, 90 °) and tilting time (1, 2 min), and then treated with prazosin (1.1-220 μg · kg- 1) and naftopidil (30 ~ 3 000 μg · kg -1) were administered in multiple doses to investigate the validity and reliability of the model. Results With the increase of tilt angle, the amplitude of blood pressure and heart rate in rats increased, and the duration of body position had no significant correlation with the change of blood pressure and heart rate. Prazosin dose-dependently enhanced orthostatic hypotension with significant inhibition of blood pressure and heart rate; naftopidil showed an inhibitory effect on blood pressure and heart rate only at a dose of 3 000 μg · kg -1. Conclusion 45 ° head-up tilt, the duration of 60 s is the most suitable condition to establish orthostatic hypotension rat model, prazosin and naftopidil test results consistent with the literature, the model proved effective and reliable.