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目的探讨过氯酸铵(ammonium perchlorate,AP)对大鼠体内碘负荷和抗氧化能力的影响。方法将SD雄性大鼠24只随机分为对照组、AP低剂量组、中剂量组和高剂量组,染毒剂量分别为130、260、520mg/kg,1次/d,每组6只,染毒13周。于第6周和第13周检测大鼠尿碘水平;并在13周后检测甲状腺组织中总碘量、相对含碘量、甲状腺组织蛋白定量、丙二醛(MDA)、超氧化物歧化酶(SOD)、甲状腺过氧化物酶(TPO)等指标。结果与对照组比较,第6周AP高剂量组尿碘水平明显升高,差异有统计学意义(P<0.05);第13周各剂量组尿碘水平与对照组比较无明显差异;各剂量组甲状腺总碘量和相对含碘量均明显低于对照组(P<0.01);AP中、高剂量组甲状腺总蛋白定量水平明显低于对照组(P<0.05);各剂量组MDA含量和SOD活力与对照组比较均无明显差异;AP高剂量组TPO活力较对照组明显升高(P<0.01)。结论AP可抑制甲状腺摄取碘,在一定时间内造成大鼠尿碘增加,并致甲状腺组织含碘量降低。AP致甲状腺蛋白定量水平减少。AP造成甲状腺过氧化物酶活力升高。
Objective To investigate the effect of ammonium perchlorate (AP) on iodine load and antioxidant capacity in rats. Methods Twenty-four SD male rats were randomly divided into control group, AP low dose group, middle dose group and high dose group. The dose of exposure was 130, 260 and 520 mg / kg, once a day, Toxic for 13 weeks. The levels of urinary iodine in rats were measured at the 6th and 13th weeks. Thirteen weeks later, the total iodine, the relative iodine content, the thyroid tissue protein, the content of malondialdehyde (MDA), superoxide dismutase (SOD), thyroid peroxidase (TPO) and other indicators. Results Compared with the control group, the level of urinary iodine in the high dose AP group was significantly increased at the 6th week (P <0.05), and the level of urinary iodine in each dose group was not significantly different from that of the control group at the 13th week. The levels of total thyroid iodine and iodine were significantly lower than those of the control group (P <0.01). The levels of total thyroid protein in AP and high dose groups were significantly lower than those in the control group (P <0.05) There was no significant difference in the activity of SOD between the control group and the high dose AP group (P <0.01). Conclusions AP can inhibit iodine uptake in the thyroid gland and increase urinary iodine in rats within a certain period of time, resulting in decreased iodine content in thyroid tissue. AP reduced thyroid protein levels. AP causes elevated thyroid peroxidase activity.