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目的:通过检测IEX-1、HIF-1α和NF-κB蛋白在宫颈癌组织和细胞中的表达情况,初步探讨其与宫颈癌发病的关系。方法:选取2009年1月~2011年10月该院病理科手术存档的蜡块标本116份,根据病变程度分为宫颈癌患者标本50份(A组)、宫颈上皮内瘤变(CIN)患者标本40份(B组)和正常宫颈标本26份(C组)。采用免疫组化法检测3组宫颈组织中IEX-1、HIF-1α和NF-κB蛋白的表达,并分析3者的相关性。结果:IEX-1在A组的阳性表达率明显低于B组和C组,差异有统计学意义(P<0.05),HIF-1α和NF-κB在A组的阳性表达率均明显高于B组和C组,差异有统计学意义(P<0.05);A组IEX-1、HIF-1α和NF-κB的表达均与组织学分级及有无淋巴结转移相关(P<0.05),与患者年龄、肿瘤的组织学类型和FIGO分期无关(P>0.05);A组HIF-1α和NF-κB的表达呈正相关(r=0.398,P<0.05),两者与IEX-1的表达呈负相关(r=-0.322、-0.517,P均<0.05)。结论:IEX-1、HIF-1α和NF-κB蛋白的表达变化与宫颈癌的发生发展有关。
OBJECTIVE: To investigate the relationship between the expression of IEX-1, HIF-1α and NF-κB in cervical cancer tissues and cells and the relationship between the expression of IEX-1 and cervical cancer. Methods: 116 paraffin-embedded specimens from January 2009 to October 2011 in our hospital were divided into group A (50 cases), cervical intraepithelial neoplasia (CIN) 40 specimens (group B) and 26 normal cervical specimens (group C). The expression of IEX-1, HIF-1α and NF-κB in 3 groups of cervical tissues was detected by immunohistochemistry, and the correlation between them was analyzed. Results: The positive expression rate of IEX-1 in group A was significantly lower than that in group B and C (P <0.05). The positive expression rates of HIF-1α and NF-κB in group A were significantly higher than those in group A (P <0.05). The expressions of IEX-1, HIF-1α and NF-κB in group A were significantly correlated with histological grade and lymph node metastasis (P <0.05), but not with group B There was a positive correlation between the expression of HIF-1αand NF-κB in group A (r = 0.398, P <0.05), the expression of both IEX-1 and IEX-1 was not correlated with histological type and FIGO stage (P> 0.05) Negative correlation (r = -0.322, -0.517, P <0.05). Conclusion: The expression changes of IEX-1, HIF-1α and NF-κB are related to the occurrence and development of cervical cancer.