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在PCR反应中,TaqDNA聚合酶是最关键的因素之一。临床使用中发现Taq酶必须和反应体系分开低温保存,否则很快失活;因此不利于运输和长期保存.如何解决以上问题,保证Taq酶的活性,一直是国内外学者关注的问题。我们根据参考文献[1,2]试验了多种方法来很高Taq酶的稳定性,结
Taq DNA polymerase is one of the most critical factors in PCR reactions. Taq enzyme was found in clinical use must be stored separately from the reaction system, or inactivated quickly; therefore not conducive to transport and long-term preservation. How to solve the above problems and ensure the activity of Taq enzyme has always been the focus of scholars at home and abroad. We tested a variety of methods to high Taq enzyme stability according to references [1, 2]