利用高密度花生基因表达谱芯片比较遗传背景相似而在产量上存在显著差异的2个南方花生品种粤油7号和汕油523荚果与叶片的基因表达谱。结果表明,汕油523与粤油7号荚果差异表达基因高达1383个,其中上调表达基因662个,下调表达基因721个。叶片中差异表达基因有647个,其中上调表达基因234个,下调表达基因413个。基因表达差异在10倍以上的上调和下调基因在荚果中分别有52个和105个,而在叶片中只有2个和5个。功能注释结果表明,差异表达基因集中在细胞内和膜上,而其分子功能主要为结合和催化活性,主要参与代谢、细胞和生物调控等生物过程。在花生荚果和叶片中,还有近一半的差异表达基因的功能未知,表明这2个器官中还有大量的基因尚待发掘。为验证基因芯片数据的可靠性和重复性,选择4个差异表达基因进行实时定量PCR分析,其结果与芯片检测结果吻合。 The gene expression profiles of two southern peanut varieties Yueyou 7 and Shanyou 523 were compared using high-density peanut gene expression profiling chips with similar genetic backgrounds but with significant differences in yield. The results showed that there were 1383 differentially expressed genes in pods of Shanyou 523 and Yueyou 7, including 662 up-regulated genes and 721 down-regulated genes. There were 647 differentially expressed genes in leaves, including 234 up-regulated genes and 413 down-regulated genes. The up-and down-regulated genes with more than 10-fold difference in gene expression had 52 and 105 pods, respectively, while there were only 2 and 5 in the leaves. Functional annotation results show that the differentially expressed genes are concentrated in the cell and the membrane, and its molecular function is mainly binding and catalytic activity, mainly involved in biological processes such as metabolism, cell and biological regulation. Nearly half of the differentially expressed genes in peanut pods and leaves are unknown, indicating that a large number of genes remain to be discovered in these two organs. In order to verify the reliability and repeatability of the gene chip data, four differentially expressed genes were selected for real-time quantitative PCR analysis, and the results were in good agreement with the chip test results.