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目的:观察血管内液体球囊放射治疗对血管成形术后再狭窄的影响,同时通过观察放射治疗效应探 讨放射治疗防止再狭窄发生的可能机制。方法:雄性Wistar大鼠72只,体重300～350g,随机分为两组,胸主动 脉经球囊损伤(损伤组),球囊损伤加32P液体球囊血管内照射(照射组,按照射剂量分为20Gy亚组和28Gy亚 组)。使用原位杂交方法测定管壁基质金属蛋白酶9(MMP 9)mRNA表达;采用免疫组化的方法测定增殖细胞 核抗原(PCNA)表达阳性细胞;用计算机图像分析方法观察血管组织形态学的变化,并对原位杂交和免疫组化的 结果进行定量分析。结果:照射组与损伤组相比:第14天外弹力板围绕面积、管腔面积明显增大,且随照射剂量 的增加而增大;第1,3,7天管壁MMP 9mRNA表达率明显降低,降低程度随照射剂量增加而增大。结论:32P液 体球囊可防止再狭窄的形成,其机制之一可能是通过抑制管壁MMP 9mRNA表达,从而抑制血管重塑。
OBJECTIVE: To observe the effect of intravascular fluid balloon radiation therapy on restenosis after angioplasty and to explore the possible mechanism of radiotherapy to prevent restenosis by observing the effect of radiation therapy. Methods: Seventy-two male Wistar rats weighing 300-350 g were randomly divided into two groups: the thoracic aorta was inflated with balloon injury (balloon injury), balloon injury with 32 P liquid balloon balloon irradiation (irradiation group, Divided into 20Gy subgroup and 28Gy subgroup). The expression of matrix metalloproteinase 9 (MMP 9) mRNA was detected by in situ hybridization. The expression of proliferating cell nuclear antigen (PCNA) was detected by immunohistochemical method. The morphological changes of vascular tissue were observed by computer image analysis The results of in situ hybridization and immunohistochemistry were quantitatively analyzed. Results: Compared with the injured group, the area around the outer elastic plate on day 14 and the luminal area increased significantly with the increase of irradiation dose. The expression of MMP 9 mRNA on the 1st, 3rd and 7th day was significantly lower , The degree of decrease increases with the increase of irradiation dose. Conclusion: 32P liquid balloon can prevent the formation of restenosis. One of the mechanisms may be that it inhibits vascular remodeling by inhibiting the expression of MMP 9 mRNA in the vascular wall.