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探讨ＨＢＶ基因变异在ＨＢｓＡｇ阴性抗ＨＣＶ阳性慢性肝病中的意义。方法用巢式聚合酶链反应（ＰＣＲ）与限制片段长度多态性相结合，对５６例慢性肝病患者６例ＨＢｓＡｇ阴性抗ＨＣＶ阳性（Ａ组）．１９例ＨＢｓＡｇ阳性抗ＨＣＶ阳性（Ｂ组）及３１例单独ＨＢＶ成染（Ｃ组）进行前Ｃ区密码２８终止变异（Ａ８３）和Ｃ区密码９７异亮氨酸亮氨酸变异（Ｌ９７）分析。结果Ａ组和Ｂ组Ａ８３和Ｌ９７变异检出率分别为３３％和４２％显著低于Ｃ组８１％（Ｐ值＜０．００１）：而Ａ组和Ｂ组间无统计学差异（Ｐ值＞０．０５）。结论ＨＢＶ和ＨＣＶ双重感染ＨＢｓＡｇ阴性与Ａ８３和Ｌ９７变异无相关。
To investigate the significance of HBV gene mutation in HBsAg negative anti HCV positive chronic liver disease. Methods Nested polymerase chain reaction (PCR) combined with restriction fragment length polymorphism (PCR-RFLP) was used to detect the presence of HBsAg-negative anti-HCV in 56 patients with chronic liver disease (group A). 19 cases of HBsAg positive anti-HCV positive (group B) and 31 cases of HBV independent staining (group C) were used to analyze the precore mutation (A83) of the front C region and the isoleucine leucine mutation (L97) . Results The positive rates of A83 and L97 mutation in group A and group B were significantly lower than those in group C (33% and 42%, respectively) (P <0.001), but no significant difference was found between group A and group B (P > 0.05). Conclusion The negative results of HBsAg in HBV and HCV infection were not related to the mutation of A83 and L97.