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目的探讨裙带菜多糖诱导人食管癌细胞TE-13凋亡的作用及其机制。方法采用MTT法分析裙带菜多糖(25、50、100μg/mL)分别作用24、48、72 h后对TE-13细胞增殖的抑制作用;用透射电镜观察25、50、100μg/mL裙带菜多糖分别作用48 h后细胞的凋亡结构变化;用流式细胞术分析TE-13细胞的凋亡率和检测Sur-vivin蛋白的表达水平。结果不同质量浓度的裙带菜多糖均可明显抑制食管癌TE-13细胞的增殖(P<0.05),其抑制作用呈质量浓度依赖性,作用24 h后的IC50为44.8μg/mL。不同质量浓度裙带菜多糖作用48 h后,均可诱导TE-13细胞凋亡,100μg/mL裙带菜多糖可诱导TE-13细胞凋亡率达70.51%,导致TE-13细胞发生凋亡特征性超微结构改变,并使TE-13细胞Survivin蛋白表达水平明显降低(P<0.01)。结论裙带菜多糖可抑制TE-13细胞增殖,诱导细胞凋亡,该作用与下调细胞Survivin蛋白表达有关。
Aim To investigate the effect of polysaccharides from Undaria pinnatifida on apoptosis of human esophageal cancer cells TE-13 and its mechanism. Methods The inhibitory effect of polysaccharides (25, 50, 100μg / mL) on the proliferation of TE-13 cells were detected by MTT assay. The effects of 25, 50, 100μg / mL polysaccharides After 48 h, the apoptotic structure of the cells was observed. The apoptosis rate of TE-13 cells and the expression of Sur-vivin protein were detected by flow cytometry. Results The proliferation of esophageal carcinoma TE-13 cells was significantly inhibited by different concentration of polysaccharides of Undaria pinnatifida (P <0.05), and its inhibitory effect was in a concentration-dependent manner. The IC50 was 44.8 μg / mL after 24 h. The apoptosis of TE-13 cells induced by 100μg / mL Undaria pinnatifida polysaccharides could induce the apoptosis rate of TE-13 cells by 70.51%, which led to the characteristic apoptosis of TE-13 cells Ultrastructural changes, and the expression of Survivin protein in TE-13 cells was significantly decreased (P <0.01). Conclusion Undaria pinnatifida polysaccharide can inhibit TE-13 cell proliferation and induce apoptosis, which is related to the down-regulation of Survivin protein expression.