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目的:研究B7-H3在口腔鳞癌组织和正常口腔黏膜组织中的表达差异,以及B7-H3对口腔鳞癌细胞生物学的影响。方法:RT-qPCR、免疫组化检测B7-H3在口腔鳞癌组织和正常口腔黏膜组织的表达差异;构建B7-H3腺病毒表达载体,感染人舌鳞癌细胞Tca8113,CCK-8、PI染色流式细胞仪检测B7-H3高表达对Tca8113细胞增殖和细胞周期的影响。采用SPSS11.0软件包对数据进行统计学处理。结果:RT-qPCR结果显示,口腔鳞癌组织B7-H3 mRNA表达为3.021±0.2310,显著高于正常口腔黏膜组织(0.6002±0.1010);与对照组比较,10、50、100感染复数组在作用24h呈现促进细胞增殖作用,S期细胞比例显著增加,72h时增殖作用最强(P<0.05);与感染复数1组比较,同时间段50、100组促细胞增殖作用和S期细胞比例显著增加(P<0.05),50与100组比较无显著差异(P>0.05)。结论:口腔鳞癌组织B7-H3mRNA和蛋白表达显著高于正常口腔黏膜组织;B7-H3高表达,可促进口腔鳞癌细胞增殖。
OBJECTIVE: To study the difference of B7-H3 expression in oral squamous cell carcinoma and normal oral mucosa and the effect of B7-H3 on the cell biology of oral squamous cell carcinoma. Methods: The expression of B7-H3 in oral squamous cell carcinoma and normal oral mucosa tissues was detected by RT-qPCR and immunohistochemistry. The B7-H3 adenovirus expression vector was constructed to infect human tongue squamous cell carcinoma Tca8113, CCK-8 and PI Flow cytometry was used to detect the effect of B7-H3 overexpression on the proliferation and cell cycle of Tca8113 cells. SPSS11.0 software package for statistical analysis of the data. Results: RT-qPCR results showed that the expression of B7-H3 mRNA in oral squamous cell carcinoma was 3.021 ± 0.2310, which was significantly higher than that in normal oral mucosa (0.6002 ± 0.1010). Compared with the control group, (P <0.05). Compared with the infected group 1, the percentage of cells in 50 and 100 groups at the same time period was significantly higher than that in S phase (P <0.05). There was no significant difference between 50 and 100 groups (P> 0.05). Conclusion: The expression of B7-H3 mRNA and protein in oral squamous cell carcinoma is significantly higher than that in normal oral mucosa. The high expression of B7-H3 can promote the proliferation of oral squamous cell carcinoma.