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我们获得“元帅”苹果花培植株后(见《中国果树》1981年第一期40、44页),即将1980年分化的植株进行增殖、诱导生根和染色体数的鉴定工作。将增殖的无根植株置于含吲哚丁酸的(1/2)MS培养基中,或先经吲哚丁酸处理再置放于(1/2)MS培养基中诱导发根后,切取根尖按下述步骤鉴定染色体倍性(见图版2):(1)用0.1％秋水仙碱溶液预处理2—3小时或用3℃左右低温预处理48小时;(2)用卡诺氏液固定48小时后,放入解离液中(95％酒精、浓盐酸,1:1V/V)离析30—50分钟;(3)置于4％铁矾溶液中媒染30—40分钟后,转入0.5％苏木精溶液中染色三个半小时,再放入45％醋酸中分色软化1小时;(4)烘烤、压片、镜检染色体
After we obtained the “marshal” apple flower cultivation plant (see “China fruit tree” 1981 the first period 40, 44 pages), the differentiation of plants in 1980 to proliferate, induction of rooting and chromosome number identification work. Proliferation of non-rooted plants with indole butyric acid (1/2) MS medium, or first by indole butyric acid and then placed in (1/2) MS medium to induce hair roots, Chop the apices and identify chromosome ploidy according to the following steps (see Figure 2): (1) Pretreatment with 0.1% colchicine solution for 2-3 hours or pre-treatment with low temperature around 3 ℃ for 48 hours; (2) After the solution is fixed for 48 hours, the solution is placed in a dissociation solution (95% alcohol, concentrated hydrochloric acid, 1: 1 V / V) for 30-50 minutes; (3) placed in a 4% alum solution for 30-40 minutes , Transferred to 0.5% hematoxylin solution for three and a half hours, and then placed in 45% acetic acid color separation softening 1 hour; (4) baking, tableting, microscopy