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目的制备一种用于对人前列腺癌PC3细胞进行体外检测的粒细胞靶向介导的磁性-荧光纳米探针。方法制备以Fe_3O_4为核、表面包被SiO_2和异硫氰酸罗丹明的具有磁性且携带红色荧光的纳米材料,将其与正常人外周血粒细胞按不同比例混合,分别孵育不同时间以检测纳米材料对粒细胞的毒性。选择最佳比例将纳米材料和粒细胞体外结合,得到粒细胞靶向介导的磁性-荧光纳米探针;将PC3细胞与正常人全血细胞分别以不同比例混合,加入上述探针,于荧光显微镜下观察探针的靶向情况。结果在实验所用的浓度和作用时间范围内,制备的纳米探针对粒细胞存活率无明显影响。镜下可见纳米探针在PC3细胞周围富集,形成“花瓣样”结构,而全血细胞周围无探针富集。结论本研究制备的磁性-荧光纳米探针不会对粒细胞本身产生明显毒性,利用粒细胞对肿瘤细胞的生物靶向作用可使纳米探针有效地靶向检测肿瘤细胞。
OBJECTIVE: To prepare a granulocyte-targeting magnetic-fluorescent nanoparticle probe for in vitro detection of human prostate cancer PC3 cells. Methods Nanomaterials with Fe3O4 as nucleus, surface-coated SiO2 and rhodamine isothiocyanate were prepared and mixed with normal human peripheral blood granulocytes in different proportions and incubated at different times to detect the nanocrystals Material toxicity to granulocytes. Select the best ratio of nanomaterials and granulocytes in vitro binding to obtain granulocyte-mediated magnetic-fluorescent nanoprobe; PC3 cells and normal human whole blood cells were mixed in different proportions, adding the above probe, in a fluorescence microscope Look under the target of the probe. Results In the experiment concentration and time range, the preparation of nano-probe granulocyte survival rate had no significant effect. Microscope shows nano-probe enrichment around the PC3 cells, forming a “petal-like” structure, and no enrichment around the whole blood cells. Conclusion The magnetic-fluorescent nano-probe prepared in this study does not produce obvious toxicity to granulocyte itself. The biological targeting of granulocytes to tumor cells can make nano-probes effectively target tumor cells.