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目的:研究褪黑素(melatonin,MT)对宫内感染胎鼠脑组织自由基的影响,探讨MT对宫内感染胎鼠脑组织的保护作用。方法:通过给孕鼠腹腔注射细菌脂多糖(lipopolysaccharide,LPS),造成宫内感染脑瘫动物模型,并行MT干预。选用妊娠第19天SD大鼠随机分为空白对照组、宫内感染组和MT处理组,宫内感染组孕鼠缓慢腹腔注射LPS500μg/kg,MT处理组孕鼠腹腔注射LPS 500μg/kg+MT 10 mg/kg。将各大组孕鼠根据注药后观察时间不同,平均分为注药后2 h、6 h、12 h 3小组,各小组4只孕鼠。各小组于相应的时间点迅速处死,于冰盒上剖腹取胎鼠脑组织,分别测定脑组织匀浆的超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活力和丙二醛(MDA)含量,HE染色观察组织病理变化,并比较各组之间的差异。结果:宫内感染组较空白对照组胎鼠脑组织中SOD、GSH-Px活力下降,MDA含量上升;同时随着感染时间的延长,上述改变亦趋于明显,差异有统计学意义。与宫内感染组相比,MT处理组能提高胎鼠脑组织中SOD、GSH-Px活力,降低MDA含量。结论:宫内感染胎鼠脑组织在宫内已出现自由基蓄积而自身清除力不足,导致胎鼠脑组织损伤,并随着感染时间的延长而脑损伤逐渐加重。褪黑素能对宫内感染胎鼠脑损伤有抗氧化和较强的自由基清除作用,有神经保护作用。
Objective: To study the effect of melatonin (MT) on the free radicals in intrauterine infection of fetal rat brain and to explore the protective effect of MT on intrauterine fetal brain tissue. Methods: The intraperitoneal injection of bacterial lipopolysaccharide (LPS) was induced in pregnant rats, resulting in intrauterine infection of cerebral palsy animal model and MT intervention. On the 19th day of gestation, SD rats were randomly divided into blank control group, intrauterine infection group and MT treatment group. Intraperitoneally infected pregnant rats were injected intraperitoneally with LPS 500μg / kg, MT group, intraperitoneal injection of LPS 500μg / kg + MT 10 mg / kg. Each group of pregnant rats according to different time after injection, divided into 2 hours after injection, 6 h, 12 h 3 groups, each group of 4 pregnant rats. The groups were sacrificed at the corresponding time points, and the brain tissue of fetal rat was taken by cesarean section on the ice box. The contents of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) Vitality and malondialdehyde (MDA) content, histological changes were observed by HE staining, and the differences between the groups were compared. Results: Compared with blank control group, the activity of SOD and GSH-Px in brain tissue of the intrauterine infection group decreased and the content of MDA increased. At the same time, the changes also tended to be obvious with the extension of infection time, the difference was statistically significant. Compared with intrauterine infection group, MT treatment group could increase the activity of SOD, GSH-Px in brain tissue of fetal rats and decrease the content of MDA. Conclusion: Intrauterine infection of fetal rat brain tissue has emerged in the womb free radical accumulation and lack of self-cleaning, resulting in fetal rat brain tissue damage, and with the extension of infection and brain injury gradually aggravated. Melatonin can inhibit intrauterine fetal brain injury with anti-oxidant and strong free radical scavenging effect, a neuroprotective effect.