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目的:以慢性心力衰竭大鼠为研究对象,测定心肌组织Bcl-2、Bax mRNA水平及其蛋白表达,以探究燧心胶囊干预心室重塑的作用机制。方法:将60只大鼠随机分为假手术组、模型对照组、地高辛组、卡托普利组、燧心胶囊低剂量组、燧心胶囊高剂量组。采用腹主动脉缩窄术建立大鼠慢性心力衰竭模型,连续给药12周。Real time PCR检测心肌细胞凋亡基因Bcl-2 mRNA、Bax mRNA水平;免疫组化法观察大鼠心肌细胞凋亡蛋白Bcl-2、Bax的表达。结果:与模型对照组比较,卡托普利组、燧心胶囊低、高剂量组大鼠Bcl-2 mRNA水平及其蛋白表达增高,Bax mRNA水平及其蛋白表达降低,Bcl-2/Bax则显著性增高,差异具有统计学意义(P<0.05或P<0.01)。其中燧心胶囊高剂量组对Bcl-2和Bax mRNA及其蛋白的调控程度较低剂量组显著(P<0.05)。结论:高剂量燧心胶囊能够调控凋亡基因Bcl-2和Bax mRNA水平及其蛋白表达,抑制心肌细胞程序性死亡,延缓心力衰竭的进程。
OBJECTIVE: To study the expression of Bcl-2 and Bax mRNA and protein in myocardium of chronic heart failure rats, in order to explore the mechanism of intervention of Sui-heart capsule on ventricular remodeling. Methods: Sixty rats were randomly divided into sham operation group, model control group, digoxin group, captopril group, Suixin capsule low dose group and Suixin capsule high dose group. A rat model of chronic heart failure was established by abdominal aorta constriction. The rats were continuously administered for 12 weeks. Real time PCR was used to detect the expression of Bcl-2 mRNA and Bax mRNA in cardiomyocytes. The expression of Bcl-2 and Bax in rat cardiomyocytes was detected by immunohistochemistry. Results: Compared with the model control group, the expression of Bcl-2 mRNA and its protein, the expression of Bcl-2 / Bax and the protein expression of Bcl-2 / Bax were decreased in captopril group and Suixin capsule group Significantly increased, the difference was statistically significant (P <0.05 or P <0.01). Among them, Suixin capsule high-dose group had significantly lower regulation of Bcl-2 and Bax mRNA and protein (P <0.05). Conclusion: High-dose Suixin Capsule can regulate the expression of Bcl-2 and Bax mRNA and protein, inhibit the apoptosis of cardiomyocytes and delay the progression of heart failure.