论文部分内容阅读
目的:探讨脓毒症期间不同免疫器官内T细胞亚群的比例和数量变化。方法:将8周龄的雌性C57BL/6小鼠随机分为脓毒症组和对照组，脓毒症组通过盲肠结扎穿刺法构建脓毒症小鼠模型，对照组为假手术组。分别于脓毒症早期(造模后24 h)和晚期(造模后16 d)通过流式细胞术检测小鼠脾脏、腋窝及腹股沟淋巴结和骨髓中CD4n +T细胞、CD8n +T细胞、CD4n +CD25n highFoxp3n +调节性T细胞(regulatory T cell，Treg)和CD4n +CD25n lowFoxp3n -效应T细胞(effector T cell，Teff)的比例，并进行细胞计数。n 结果:小鼠脾脏中CD4n + T细胞、CD8n +T细胞于造模后24 h显著减少，且至造模后16 d仍未恢复；Teff的比例和绝对数在脓毒症期间始终处于显著减少状态，而Treg数量早期无明显变化，但于晚期显著增加。脓毒症小鼠淋巴结中CD4n + T细胞、CD8n +T细胞、Teff的比例和绝对数变化同脾脏基本一致；但Treg数量的变化与脾脏不同：早期无明显变化而晚期显著减少。此外，造模后24 h小鼠骨髓中CD4n + T细胞、CD8n +T细胞及Teff的绝对数均无显著变化，但于造模后16 d均显著减少；骨髓Treg在脓毒症期间比例和绝对数均显著减少。n 结论:脓毒症不同时期，主要免疫器官内的淋巴细胞大量消耗，多数情况下Treg与其他T细胞具有不同的变化特点。主要免疫器官间T细胞的变化存在差异，提示不同免疫器官对脓毒症的反应具有异质性。“,”Objective:To explore the changes in the proportion and number of T cell subsets in different immune organs during sepsis.Methods:Eight-week-old female C57BL/6 mice were randomly divided into sepsis group and sham group.The experimental sepsis model was constructed through cecal ligation and puncture, and the sham group just underwent sham operation.Then we detected the changes in the total number of lymphocytes and in the ratio and absolute number of CD4n + T cells, CD8n + T cells, CD4n + CD25n high Foxp3n + regulatory T cells(Treg) and CD4n + CD25n low Foxp3n - effector T cells(Teff) in the mouse spleen, axillary and inguinal lymph nodes and bone marrow by cell counting and flow cytometry 24 h and 16 d after modeling.n Results:In the spleens of septic mice, the ratio and absolute numbers of CD4n + T cells and Teff, as well as the absolute number of CD8n + T cells were significantly reduced 24 h and 16 d after modeling.There was no significant change in the number of Treg 24 h after modeling, but a significant increase occurred 16 d after modeling.During sepsis, the changes of CD4n + T cells, CD8n + T cells and Teff in mouse lymph nodes were basically the same as those in the spleen; but the changes in Treg were different, with no significant change in the early stage and a significant decrease in the late stage.In addition, the absolute numbers of CD4n + T cells, CD8n + T cells, and Teff in the bone marrow did not change significantly in the 24 h model, but decreased significantly in the 16 d model.The proportion and absolute number of Treg during sepsis were significantly reduced.n Conclusion:During different periods of sepsis, there is a large consumption of lymphocytes in the spleen, lymph nodes and bone marrow.In most cases, the trend of Treg changes is inconsistent or even opposite to that of other T cell subsets.There are differences in the changes of T cells among major immune organs, suggesting that the responses of different immune organs to sepsis are heterogeneous.