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建立了犬血浆和小鼠组织、尿和粪中紫杉醇的ＨＰＬＣ检测法。生物样品用乙醚提取后在Ｃ１８柱上以０．０１ｍｏｌ／Ｌ磷酸二氢钠－甲醇（４０６０，ｐＨ＝４．３）为流动相，安定为内标，检测波长为２３３ｎｍ条件下进行检测。线性范围为１８．７～３６００μｇ／Ｌ，最低检测浓度为１０μｇ／Ｌ。紫杉醇在犬血浆和小鼠肝、尿、粪中的回收率分别为８６．０４％～９１．７３％，８７．６２％～９２．１２％，８６．７８％～９２．１６％，７９．８３％～８５．６２％；日内ＲＳＤ分别为２．３３％～３．７８％，１．３７％～４．５５％，３．５４％～４．２８％，２．３０％～４．０６％；日间ＲＳＤ分别为３．３１％～４．３８％，４．７２％～５．５１％，６．７５％～８．５３％，４．４３％～６．６７％。用本法对家犬ｉｖ紫杉醇后的药代动力学进行了研究，并报告了有关的动力学参数。
A HPLC method for the determination of paclitaxel in dog plasma, mouse tissues, urine and feces was established. The biological samples were extracted with ether and detected on the C18 column with 0.01 mol / L sodium dihydrogen phosphate-methanol (4060, pH = 4.3) as the mobile phase and stable as the internal standard at a detection wavelength of 233 nm. The linear range of 18.7 ~ 3600μg / L, the lowest detection concentration of 10μg / L. The recoveries of paclitaxel in liver, urine and feces from dogs and cats were 86.04% -91.73%, 87.62% -92.12% and 86.78% -92.16%, respectively. 83% ~ 85.62%. The intra-day RSD was 2.33% -3.78%, 1.37% -4.55%, 3.54% -4.28%, 2.30% ~ 4.06 %; Daytime RSD were 3.31% ~ 4.38%, 4.72% ~ 5.51%, 6.75% ~ 8.53%, 4.43% ~ 6.67% respectively. Pharmacokinetics of paclitaxel in domestic dogs was studied using this method and related kinetic parameters were reported.