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目的探讨荧光定量聚合酶链反应(FQ-PCR)在检测乙型肝炎病毒(HBV)和抗病毒疗效评价方面的临床意义。方法采用 FQ-PCR、ELISA、常规 PCR 定性方法检测532例慢性乙型肝炎患者血清。结果 240例乙肝“大三阳”患者中,常规 PCR 定性均为阳性,阳性率100%;FQ-PCR 检出236例为阳性,阳性经为98.3%;140例乙肝“小三阳”患者中,常规 PCR 定性有62例阳性,阳性率为44.3%,FQ-PCR 检出51例阳性。阳性率为36.4%;80例乙肝“小二阳”患者中,常规 PCR 定性有32例阳性,阳性率为40%,FQ-PCR 检出26例阳性,阳性率为32.5%;42例乙肝 HBeAb 和 HBcAb 阳性患者者,常规 PCR 定性有15例阳性,阳性率为35.7%,FQ-PCR检出10例阳性,阳性率为23.8%;30例乙肝 HBsAb、HBeAb、HBcAb 三项阳性的患者中,常规 PCR 定性有8例阳性,阳性率为26.7%,FQ～PCR 检出5例阳性,阳性率为16.7%。结论 FQ-PCR 在检测乙肝病毒复制方面的准确性明显高于 ELISA 法和常规 PCR 定性方法。
Objective To investigate the clinical significance of fluorescent quantitative polymerase chain reaction (FQ-PCR) in the detection of Hepatitis B virus (HBV) and anti-virus efficacy evaluation. Methods The serums of 532 patients with chronic hepatitis B were detected by FQ-PCR, ELISA and routine PCR methods. Results Of the 240 patients with hepatitis B and Sansheng Yang, all the patients were positive by conventional PCR and the positive rate was 100%. 236 cases were positive by FQ-PCR and 98.3% Among the patients, 62 cases were positive by conventional PCR, the positive rate was 44.3%, and 51 cases were positive by FQ-PCR. The positive rate was 36.4%. Among 80 cases of hepatitis B patients, 32 cases were positive by routine PCR, the positive rate was 40%, 26 cases were positive by FQ-PCR, the positive rate was 32.5%; 42 cases Among HBeAb and HBcAb positive patients, 15 cases were positive by conventional PCR, the positive rate was 35.7%, 10 cases were positive by FQ-PCR, the positive rate was 23.8%. Thirty patients with HBsAb, HBeAb and HBcAb positive Among them, 8 cases were positive by conventional PCR, the positive rate was 26.7%, 5 cases were positive by FQ ~ PCR, the positive rate was 16.7%. Conclusion The accuracy of FQ-PCR in detecting hepatitis B virus replication was significantly higher than that of ELISA and conventional PCR.