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大豆细菌性斑点病是严重危害大豆生产的叶部病害。本研究采用针刺法,对高抗大豆细菌性斑点病4号生理小种的大豆品种绥农8和高感品种合丰25分别接种大豆细菌性斑点病4号生理小种后,进行透射电镜观察。结果表明,随着接种时间的增加,抗感大豆品种叶片超微结构出现了明显差异,接种120h后,抗病品种叶片细胞内部结构基本上比较完整,而感病品种叶片细胞内部结构发生了较大的变化,细胞质膜、核膜、核质及叶绿体均受到严重的破坏;叶绿体基粒片层消失,大部分叶绿体膜也逐渐解体,线粒体的空泡化严重,说明大豆细菌性斑点病引起细胞结构病变在抗感品种间的表现不同。研究为揭示大豆细菌性斑点病病原菌的致病机理奠定一定的理论基础。
Soybean bacterial speckle is a disease of leaves that severely compromises soybean production. In this study, the acupuncture method was used to study the effects of Soybean bacterial speckle disease resistant variety Suinong 8 and Hefeng 25, a variety resistant to soybean bacterial spot disease 4, Observed. The results showed that with the increase of inoculation time, the ultrastructure of leaves of resistant soybean showed obvious difference. After 120 h of inoculation, the internal structure of leaves of resistant varieties was basically complete, while the internal structure of susceptible varieties of leaves occurred more Large changes in the plasma membrane, nuclear membrane, nuclear and chloroplasts were severely damaged; chloroplast glabrous disappeared, most of the chloroplast membrane also gradually disintegrated, mitochondria vacuolization serious, indicating that soybean bacterial speckle disease caused by cells Structural lesions varied among resistant varieties. The research laid a theoretical foundation for revealing the pathogenesis of bacterial pathogen of soybean bacterial spot.