目的对云南省野鼠鼠疫疫源地齐氏姬鼠盲肠内容物中分离的1株鼠疫噬菌体(L128m)进行鉴定,探讨鼠疫自然疫源地微生态因素保存鼠疫自然疫源性发挥的作用。方法以鼠疫疫苗株EV 76为宿主菌,采用双层琼脂平皿法从云南省鼠疫疫源地齐氏姬鼠盲肠中分离1株温和噬菌体;描述其形态特征;测定裂解能力、宿主谱。结果鼠疫噬菌体L128m形成的噬菌斑中心和边缘均模糊,直径为0.9~1.0 mm,其为溶原性噬菌体;通过液体增殖培养后其效价达到106~P FU/ml;电镜观察其形态呈蝌蚪形,头部为正多面体结构,头部直径约为61 nm,尾部长约155 nm,带有一伸缩的尾鞘,归类为肌尾噬菌体。宿主谱评价发现仅裂解鼠疫疫苗株。结论从1只齐氏姬鼠盲肠内容物中分离的鼠疫噬菌体属溶原性噬菌体,它的发现对我国鼠疫疫源地宿主动物肠道微生态学和流行病学研究具有重要意义。 Objective To identify one isolate of plague phage (L128m) isolated from the cecal contents of Alzheimer ’s disease rats in the plague origin of Yunnan Province to investigate the role of the natural foci of plague in the natural foci of plague. Methods The plague vaccine strain EV 76 was used as the host strain. A double-layer agar plate method was used to isolate a mild phage from the cecum of Alzheimer’s disease rats in Yunnan plague origin. Its morphological characteristics were described. The lysis capacity and host spectrum were determined. Results The phage plaque formed by the plague phage L128m was fuzzy and centered at 0.9-1.0 mm in diameter, which was a lysogenic bacteriophage. The titer reached 106 ~ P FU / ml after being cultured by liquid, and its morphology was observed by electron microscopy Tadpole shape, the head is a positive polyhedron structure, the head diameter of about 61 nm, the tail length of about 155 nm, with a retractable tail sheath, classified as myogenic phage. Host spectrum evaluation found that only plague vaccine strains were split. CONCLUSION: The phage plague bacteriophage isolated from the cecal contents of one zygophila is an important lysogenic bacteriophage. Its discovery is of great significance to the study of intestinal microecology and epidemiology of host animals in the plague origin in our country.