论文部分内容阅读
目的:对野坝子Elsholtzia rugulosa Hemsl.乙醇提取物的不同极性萃取部位和AB-8大孔树脂柱色谱不同浓度甲醇洗脱部位清除自由基能力进行综合评价。方法:分别以维生素C和维生素E为对照,采用清除2,2-二苯基苦味酰基苯肼基(2,2-diphenyl-1-picrylhydrazyl,DPPH)和2,2′-连氮基-双-(3-乙基苯并噻唑啉-6-磺酸)二铵盐[2,2′-azino-bis(3-ethylbenzthiazo-line-6-sulphonic acid)diammonium salt,ABTS]自由基的方法,通过计算半数抑制浓度评价野坝子的乙醇提取物的不同极性萃取部位和AB-8大孔树脂柱色谱不同浓度甲醇洗脱部位的清除自由基能力。结果:野坝子提取物不同极性萃取部位清除DPPH自由基和ABTS自由基的能力分别为:乙酸乙酯部位>Vit C>正丁醇部位>水部位>石油醚部位>氯仿部位、乙酸乙酯部位>Vit E>正丁醇部位>水部位>氯仿部位>石油醚部位。AB-8大孔树脂柱色谱不同浓度甲醇洗脱部位清除DPPH和ABTS自由基的能力分别为Vit C>50%甲醇部位>70%甲醇部位>30%甲醇部位>100%甲醇部位、50%甲醇部位>70%甲醇部位>30%甲醇部位>Vit E>100%甲醇部位。结论:野坝子乙醇提取物的乙酸乙酯萃取部位和30%,50%和70%甲醇洗脱部位都具有很好的清除自由基活性,是一种新型天然抗氧化物质来源。
OBJECTIVE: To evaluate the ability of free radical scavenging of Elsholtzia rugulosa Hemsl ethanol extract at different polarity and AB-8 macroporous resin column with different concentrations of methanol. Methods: Vitamin C and vitamin E were used as control, respectively, to remove 2,2-diphenyl picrylhydrazyl (2,2-diphenyl-1-picrylhydrazyl, DPPH) and 2,2’- - (3-ethylbenzthiazo-line-6-sulphonic acid) diammonium salt (ABTS) The half-inhibitory concentration was used to evaluate the free radical scavenging ability of different concentrations of ethanol extracts from Yebaizi and the elution fractions of different concentrations of methanol in AB-8 macroporous resin column chromatography. RESULTS: The capacities of the extracts of Radix Bupleuri and its extracts with different polarities were DPPH radical scavenging activity and ABTS free radical scavenging activity respectively: ethyl acetate> Vit C> n-butanol> water> petroleum ether> chloroform, ethyl acetate Site> Vit E> n-butanol> water> chloroform> petroleum ether. AB-8 macroporous resin column chromatography with different concentrations of methanol eluted parts of DPPH and ABTS free radical scavenging capacity were Vit C> 50% methanol> 70% methanol> 30% methanol> 100% methanol, 50% methanol > 70% methanol> 30% methanol> Vit E> 100% methanol. CONCLUSION: The extract of Yebaizi has the best free radical scavenging activity at the ethyl acetate extraction site and 30%, 50% and 70% methanol elution sites. It is a new source of natural antioxidants.