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利用荧光AFLP标记技术对来自秦巴山区的野板栗10个居群共262个单株进行遗传多样性研究。10对AFLP引物共扩增出1297条谱带,其中多态性位点数1011个,多态位点百分率为77.95%;Ne′is基因多样性指数为0.1439~0.2046,总体为0.2518;Shannon信息指数的变异范围为0.1972~0.2895,总体为0.4089;甘肃地区野板栗居群遗传多样性水平最高,陕西宝鸡居群的遗传多样性水平最低。AMOVA分析表明野板栗居群间的遗传变异占总变异的17.51%,居群内变异占69.76%。UPGMA聚类可将供试10个居群划分为3类,聚类结果表现出明显的地域性。
A total of 262 individuals of 10 populations of wild chestnut from Qinba Mountain were studied using fluorescent AFLP markers for genetic diversity. 10 AFLP primers amplified a total of 1297 bands, of which 1011 polymorphic loci, the percentage of polymorphic loci was 77.95%; Ne’is gene diversity index was 0.1439 ~ 0.2046, overall 0.2518; Shannon Information Index The variation range was 0.1972 ~ 0.2895 and the population was 0.4089. The genetic diversity of wild chestnut was the highest in Gansu and the lowest in Shaanxi Baoji. The AMOVA analysis showed that the genetic variation among wild chestnut populations accounted for 17.51% of the total variance, and the intra-population variation accounted for 69.76%. UPGMA clustering can divide ten populations of the test into three categories, and the clustering results show obvious regional characteristics.