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利用内皮细胞流动小室方法对大鼠脑微血管内皮细胞在剪切力作用下细胞核内原癌基因c -fos蛋白的翻译水平的表达进行了初步研究 ,结果提示脑微血管内皮细胞在剪切力作用下 ,c -fos蛋白有明显的表达且显示了剪切力水平的非依赖性和作用时间的依赖性。流动剪切力诱导的c -fos的适度表达可以导致内皮细胞伸展、细胞骨架蛋白合成、细胞骨架重排等 ,以适应流动剪切力的作用。但过度的剪切力作用可以引起内皮细胞皱缩、损伤、脱落 ,这时的c-fos会有过度的表达。如何控制c -fos蛋白的表达量 ,可能是阻断内皮细胞损伤的可能途径之一。该工作为进一步开展剪切力对微血管内皮细胞信号转导机制的影响提供了实验数据。
Using endothelial cell flow chamber method to study the translational expression of c-fos protein in rat brain microvascular endothelial cells under the action of shear stress, the results suggest that under the action of shear force, The c-fos protein is clearly expressed and shows an independent and time-dependent dependence of shear force levels. Flow-induced shear stress-induced c-fos moderate expression can lead to endothelial cell expansion, cytoskeletal protein synthesis, cytoskeletal rearrangement, etc., to adapt to the role of flow shear force. However, excessive shear force can cause endothelial cell shrinkage, injury, loss, when c-fos will have excessive expression. How to control the expression of c-fos protein may be one of the possible ways to block the damage of endothelial cells. This work provided experimental data for further study on the effect of shear stress on the signal transduction mechanism of microvascular endothelial cells.