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目的:分析结核分枝杆菌(MTB)抗原Rv2389的T细胞表位,确定和筛选优势表位,为研制更加有效的诊断标志物,和更安全、高效的表位疫苗奠定基础。方法:应用在线预测软件IEDB和SYFPEITHI对MTB抗原Rv2389的T细胞表位进行预测,并与ESAT-6相比较;采用SOPMA Sever软件预测其编码蛋白的二级结构;用Ex PASy在线软件预测Rv2389的三级结构,综合分析Rv2389的T细胞抗原决定簇。结果:经软件分析,Rv2389多肽预测分值普遍高于ESAT-6,Rv2389分值较高的T细胞表位区域氨基酸位于35~43、85~93、107~126和184~200。MTB抗原Rv2389蛋白无规则卷曲占71.89%,β折叠占5.53%,主要覆盖的氨基酸区域位14~23,51~67,72~112,117~127和134~212。说明这些肽段存在潜在的抗原表位优势区域的可能性;三级结构预测显示,85~93位氨基酸和107~126位氨基酸暴露于蛋白表面,是最有可能的抗原表位。结论:经生物信息学软件预测MTB抗原Rv2389有2个T细胞优势表位,即85~93位和107~126位氨基酸。
OBJECTIVE: To analyze the T cell epitopes of Mycobacterium tuberculosis (MTB) antigen Rv2389 and to identify and screen the dominant epitopes for the development of more effective diagnostic markers and safer and more efficient epitope vaccines. Methods: The online prediction software IEDB and SYFPEITHI were used to predict the T cell epitopes of MTB antigen Rv2389 and compared with ESAT-6. SOPMA Sever software was used to predict the secondary structure of the protein. The prediction of Rv2389 Tertiary structure, a comprehensive analysis of Rv2389 T cell epitopes. Results: The predicted value of Rv2389 polypeptide was generally higher than that of ESAT-6 by software analysis. The amino acid of T cell epitope region with high Rv2389 score was 35-43, 85-93, 107-126 and 184-200. The irregular curl of the MTB antigen Rv2389 protein accounted for 71.89% and the β-sheet folded accounted for 5.53%. The major amino acid region of the MTB antigen was 14-23, 51-67, 72-112, 117-127 and 134-212. Indicating the possibility that these peptides have potential epitopes. The tertiary structure prediction shows that amino acids 85-93 and amino acids 107-126 are the most probable epitopes upon exposure to the protein surface. Conclusion: The bioinformatics software predicts that MTB antigen Rv2389 has two dominant epitopes of T cells, namely amino acids 85-93 and 107-126.