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临床为了观察分离出的隐球菌的毒力,以鉴别是否为新型隐球菌(以下简称新隐),或制备动物感染模型以供教学或药物研究,均需进行动物接种,但对接种的某些问题尚无一致意见。现将本科真菌室对小白鼠不同途径接种的实验结果报道如下。 1 材料与方法 将从患者分离鉴定后的新隐,转种于沙博氏琼脂37℃孵育3天,用生理盐水制备成每ml约50万个孢子的悬液。实验动物为本校动物室自繁杂种小白鼠,按接种途径分颅内、尾静脉和腹腔3组,各10只。
Clinical In order to observe the virulence of isolated Cryptococcus to identify whether Cryptococcus neoformans (hereinafter referred to as Xin Yin), or preparation of animal models of infection for teaching or drug research, are required for animal vaccination, but some of the vaccination There is no consensus on the issue. Now undergraduate fungal room of different routes of mice vaccination experimental results are reported below. MATERIALS AND METHODS Separation and identification of new implants from patients were carried out and the seeds were seeded onto Sabouraud agar for 3 days at 37 ° C. A suspension of approximately 500,000 spores per ml was prepared with physiological saline. Experimental animals for the school animal room since the hybrid mice, according to the route of vaccination sub-intracranial, tail vein and abdominal cavity 3 groups, each 10.