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目的探讨CYP11A1基因rs900798位点多态性与老年性骨质疏松性骨折骨转换标志物的关系。方法研究对象为121例老年性骨质疏松性骨折患者(骨折组),114例老年性骨质疏松症患者(对照组)。骨折组中胸椎骨折、腰椎骨折、胸腰椎骨折、股骨颈骨折、粗隆间骨折、肱骨近端骨折和桡骨远端例数分别为32例、40例、3例、19例、20例、4例和3例。采用SNa Pshot法进行SNP分型,化学发光法测定血清Ⅰ型前胶原氨基端前肽(PINP)、Ⅰ型胶原羧基端肽β特殊序列(β-CTX)浓度。计算两组等位基因频率、基因型频率并分析骨折组CYP11A1基因多态性与PINP、β-CTX的关系。结果两组rs900798位点等位基因基因频率和基因型频率分布符合哈迪温伯格平衡。骨折组和对照组G、T等位基因频率分别为40.1%、59.9%和38.2%、61.8%,差异无统计学意义(P>0.05);两组GG、GT、TT基因型频率分别为14.9%、50.4%、34.7%和13.2%、50.0%、36.8%,差异无统计学意义(P>0.05)。骨折组和对照组血清PINP、β-CTX差异无统计学意义(P>0.05)。骨折组中GG、GT、TT基因型的PINP、β-CTX差异无统计学意义(P>0.05)。结论 CYP11A1基因rs900798位点多态性与老年性骨质疏松性骨折患者血清PINP、β-CTX无相关性。
Objective To investigate the relationship between rs900798 polymorphism of CYP11A1 gene and bone turnover markers in senile osteoporotic fractures. Methods The subjects were 121 senile osteoporotic fracture patients (fracture group) and 114 senile osteoporosis patients (control group). Thoracic fractures, lumbar fractures, thoracolumbar fractures, femoral neck fractures, intertrochanteric fractures, proximal humerus fractures and distal radius were 32, 40, 3, 19, 20, and 4 respectively Cases and 3 cases. SNP typing was performed by SNa Pshot method. The chemiluminescence method was used to determine the concentration of serum type Ⅰ procollagen peptide (PINP) and type Ⅰ collagen β-terminal peptide (β-CTX). The frequencies of alleles and genotypes in two groups were calculated and the relationship between CYP11A1 gene polymorphism and PINP and β-CTX in fracture group was analyzed. Results The allele frequency and genotype distribution of rs900798 in both groups were in accordance with Hardin Weinbalance. The frequencies of G and T alleles in fracture group and control group were 40.1%, 59.9% and 38.2%, 61.8% respectively, with no significant difference (P> 0.05). The frequencies of GG, GT and TT genotypes were 14.9 %, 50.4%, 34.7% and 13.2%, 50.0% and 36.8%, respectively, with no significant difference (P> 0.05). The difference of PINP and β-CTX in fracture group and control group was not statistically significant (P> 0.05). There were no significant differences in PINP and β-CTX between GG, GT and TT genotypes in fracture group (P> 0.05). Conclusion There is no correlation between CYP11A1 rs900798 polymorphism and serum PINP and β-CTX in senile osteoporotic fracture patients.