目的探讨丙型肝炎病毒HCV包膜基因E1E2,对核心基因CDNA疫苗诱生的免疫应答有无增强作用。方法构建包含HCVC或CE1E2基因片段的真核表达载体pHCVC和pHCVCE1E2,分别接种于Balb/c小鼠股四头肌以空载体pcDNA3作为对照,每间隔2wk加强免疫1次。用ELISA法检测免疫小鼠血清中抗HCVC特异性抗体的产生。以pHCVC转染并表达HCcAg的Sp2/0细胞为靶细胞,采用51Cr释放试验检测特异性CTL的杀伤作用。结果两个实验组免疫的20只小鼠均产生抗HCVC特异性抗体,当效/靶细胞比例为100∶1时,CTL的杀伤率均明显高于对照组P0.01;而pHCVCE1E2与pHCVC组之间,无论是抗HCVC抗体的滴度还是CTL的杀伤率均无显著性差异P0.05。结论E1E2基因的加入,并没有增加HCVC基因DNA疫苗诱导的抗HCcAg特异性抗体的滴度和CTL的杀伤作用。 Objective To investigate whether HCV envelope gene E1E2 of hepatitis C virus enhances the immune response induced by CDNA vaccine of core gene. Methods Construction of eukaryotic expression vector pHCVC and pHCVCE1E2 containing HCVC or CE1E2 gene fragment were inoculated into empty quadruplet of Balb / c mice with empty vector pcDNA3 as control, and immunization was performed once every 2 weeks. The production of anti-HCVC-specific antibodies in the serum of immunized mice was detected by ELISA. Sp2 / 0 cells transfected with pHCV-C and expressing HCcAg were used as target cells. 51Cr release assay was used to detect the killing effect of specific CTLs. Results Twenty mice immunized with the two experimental groups all produced anti-HCVC specific antibodies. When the ratio of target cells to target cells was 100: 1, the killing rates of CTL were significantly higher than those of the control group (P <0.01) Between pHCV C group, no significant difference in anti-HCVC antibody titers or CTL killing rate was no significant difference P <0.05. Conclusion The addition of E1E2 gene did not increase the titer of anti-HCcAg-specific antibody induced by HCVC DNA vaccine and CTL killing effect.