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本文报道采用Act-Ultrogel AcA 22亲和层析柱从胎儿尸体匀浆物内提取人体甲胎蛋白(αFP)的方法。此法费时短,收率较高,纯度和抗原性较好。提取的αFP用于~(125)I标记,可获得较好免疫活性的~(125)I-αFP,该标记品可用于αFP放射免疫分析。
This article reports the method of extracting human alpha-fetoprotein (αFP) from fetal cadaver homogenates using an Act-Ultrogel AcA 22 affinity column. This method is time-consuming, high yield, purity and antigenicity better. The extracted αFP was used in ~ (125) I labeling to obtain the ~ (125) I-αFP with better immunocompetence, which could be used for αFP radioimmunoassay.