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目的探讨丹黄通脉方对颈动脉粥样硬化斑块的影响及可能作用机制。方法 30只SD大鼠随机分为空白组、模型组、丹黄通脉方组,每组10只。模型组、丹黄通脉方组采用高脂复合饲料及腹腔注射维生素D3法制作颈动脉粥样硬化模型。造模成功后丹黄通脉方组给予丹黄通脉方10 ml/(kg·d)灌胃,空白组及模型组给予等量蒸馏水,每日2次,连续6周。观察各组大鼠颈动脉病理形态学变化,测定血清基质金属蛋白酶9(MMP-9)、基质金属蛋白酶抑制剂1(TIMP-1)水平。结果光镜下观察显示,模型组血管内膜明显增厚,泡沫样细胞堆积,弹力纤维层不同程度破坏、断裂。丹黄通脉方组血管内膜稍增厚,可见少量泡沫样细胞形成,弹力纤维层稍紊乱,程度较模型组轻。与空白组比较,模型组和丹黄通脉方组颈动脉内膜、中膜面积比(I/M)、MMP-9、TIMP-1均明显升高(P<0.05);与模型组比较,丹黄通脉方组大鼠I/M、血清MMP-9水平明显降低(P<0.05或P<0.01)。结论丹黄通脉方能够下调MMP-9水平,可能是改善颈动脉粥样硬化和稳定斑块的作用机制之一。
Objective To explore the effect of Danhuang Tongmai on carotid atherosclerotic plaque and its possible mechanism. Methods Thirty SD rats were randomly divided into blank group, model group and Danhuang Tongmai group, with 10 rats in each group. Model group and Danhuang Tongmai formula group were given carotid atherosclerosis model by high-fat compound diet and intraperitoneal injection of vitamin D3. After the model was established, Danhuang Tongmai Fang group was given Danhuang Tongmai prescription of 10 ml / (kg · d) orally. The blank group and the model group were given equal volume of distilled water twice a day for 6 weeks. The pathological changes of the carotid arteries in each group were observed. The levels of serum MMP-9 and TIMP-1 were measured. Results The light microscopic examination showed that the intima of the model group was obviously thickened, the foam-like cells were accumulated, and the elastic fiber layer was damaged and ruptured to varying degrees. Dan Huang Tongmai group of vascular intima slightly thicker, showing a small amount of foam-like cells, elastic fiber layer slightly disturbed, the degree of light than the model group. Compared with the blank group, the carotid artery intima-media thickness ratio (I / M), MMP-9 and TIMP-1 in model group and Dan Huang Tongmai Fang group were significantly increased (P <0.05) , And the I / M and serum MMP-9 levels in Danhuang Tongmai Fang group were significantly decreased (P <0.05 or P <0.01). Conclusion Dan Huang Tong Mai can down-regulate the level of MMP-9, which may be one of the mechanisms of improving carotid atherosclerosis and stable plaque.