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目的探讨不同给氧浓度对大鼠心肺复苏(CPR)结局的影响。方法选取成年雄性SD大鼠32只,按随机原则分成对照组与复苏组(包括21%氧浓度组、50%氧浓度组及100%氧浓度组),每组8只。对照组仅行麻醉、气管插管和血管穿刺。复苏组建立大鼠心脏骤停(CA)/CPR模型,分别给予21%、50%和100%浓度的氧气。采用ELISA法检测每组复苏成功后0.5、3、6 h大鼠血清S100β蛋白、神经元特异性烯醇化酶(NSE)水平。结果与对照组相比,复苏组大鼠血清S100β蛋白水平均于复苏成功后逐渐升高(P<0.05),并于复苏后3 h达到峰值;复苏组大鼠NSE水平随时间增加逐渐升高(P<0.05)。复苏组组间对比:21%氧浓度组大鼠血清S100β蛋白、NSE水平升高最为明显,100%氧浓度组次之,50%氧浓度组升高程度最低,差异有统计学意义(P<0.05)。结论给予50%浓度氧气较100%、21%浓度氧气更能降低CPR大鼠血清S100β蛋白和NSE水平,并减轻CPR后的继发性脑损伤,从而改善脑功能。
Objective To investigate the effects of different oxygen concentration on the outcome of cardiopulmonary resuscitation (CPR) in rats. Methods Thirty - two adult male Sprague - Dawley rats were randomly divided into control group and resuscitation group (including 21% oxygen group, 50% oxygen group and 100% oxygen group) with 8 rats in each group. Control group only anesthesia, endotracheal intubation and vascular puncture. Resuscitation group established rat cardiac arrest (CA) / CPR model, respectively, given 21%, 50% and 100% concentration of oxygen. Serum levels of S100β and neuron-specific enolase (NSE) were detected by ELISA at 0.5, 3 and 6 h after successful resuscitation in each group. Results Compared with the control group, the serum S100β level in the resuscitation group increased gradually after resuscitation (P <0.05), and peaked at 3 h after resuscitation. The NSE level in the resuscitation group increased gradually with time (P <0.05). Compared with the recovery group, the serum S100β protein and NSE level increased most significantly in 21% oxygen group, followed by that in 100% oxygen group and the lowest in 50% oxygen group (P < 0.05). Conclusions Oxygen of 50% concentration is more effective than 100% oxygen and 21% oxygen can reduce the levels of S100β protein and NSE in CPR rats and relieve secondary brain injury after CPR, thus improving brain function.