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目的:监测2009年/2010年北京市和天津市2地在校学生流感嗜血杆菌携带情况。方法:选择北京市和天津市大中小学生上呼吸道感染患者作为监测对象,利用PCR分子生物学技术,对急性上呼吸道患者临床标本进行流感嗜血杆菌核酸检测和血清型分型。结果:共调查了154例上感病例,其中北京84例,天津70例。北京病例标本中共检出流感嗜血杆菌80例,天津病例标本中共检出流感嗜血杆菌61例。141例阳性标本中,非荚膜型106例,荚膜型35例。35例荚膜型中,c型28例,d型1例,另外6例未能分型。结论:北京和天津2地学生主要携带非荚膜型流感嗜血杆菌,携带的荚膜型菌株主要为c型流感嗜血杆菌。
Objective: To monitor the status of Haemophilus influenzae in two schools in Beijing and Tianjin in 2009/2010. Methods: Patients with upper respiratory tract infection in primary and secondary school students in Beijing and Tianjin were selected as the monitoring subjects. The clinical samples of patients with acute upper respiratory tract were detected by DNA molecular typing and genotyping of Haemophilus influenzae. Results: A total of 154 cases were investigated, including 84 in Beijing and 70 in Tianjin. Eighty cases of Haemophilus influenzae were detected in Beijing cases and 61 cases of Haemophilus influenzae were detected in Tianjin cases. Among the 141 positive samples, there were 106 cases of non-capsule type and 35 cases of capsule type. Of the 35 capsule types, 28 were type c, one was type d and the other 6 were not typed. Conclusion: The two students in Beijing and Tianjin mainly carry non-capsular influenza Haemophilus influenzae, the main capsular strains carried are Haemophilus influenzae type c.