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目的:研究3,5-二硝基水杨酸(DNS)法测定羟乙基淀粉还原端的条件。方法:以葡萄糖做标准曲线来测定羟乙基淀粉还原端的含量,研究DNS试剂的用量、加热温度、加热时间及反应后放置时间对测定结果的影响。结果:DNS法测定羟乙基淀粉的最佳反应条件是:DNS试剂用量0.8 ml,反应温度85℃,反应时间5 min,显色定容后于540 nm的波长处测定吸光度。结论:本方法操作简单、准确,重复性好,可用于羟乙基淀粉还原端的测定。
Objective: To study the conditions of 3,5-dinitrosalicylic acid (DNS) determination of hydroxyethyl starch reducing end. Methods: The standard curve of glucose was used to determine the reducing end of hydroxyethyl starch. The effects of DNS reagent dosage, heating temperature, heating time and reaction time on the determination results were studied. Results: The optimum reaction conditions for the determination of hydroxyethyl starch by DNS method were as follows: the amount of DNS reagent was 0.8 ml, the reaction temperature was 85 ℃, the reaction time was 5 min, and the absorbance was measured at the wavelength of 540 nm. Conclusion: The method is simple, accurate, reproducible and can be used for the determination of hydroxyethyl starch reducing end.