建立骨肉瘤细胞系,有利于对骨肉瘤生物学特性的深入研究。我们从1985年12月开始共培养了11例骨肉瘤手术切除标本,其中1例在体外培养建系获得成功。从1986年4月19日至今连续传代培养40余代,命名为HOS-8603,现将该细胞系的建立过程及其生物学特性作一简要报道。 1 材料与方法 1.1 材料 我们于1986年4月19日从我校长海医院取得骨肉瘤手术切除标本,患者男性,23岁, The establishment of osteosarcoma cell lines is conducive to in-depth study of biological characteristics of osteosarcoma. From December 1985, we co-cultured 11 cases of osteosarcoma resected specimens, of which 1 case of in vitro culture line was successful. From April 19, 1986 to now, it has been continuously subcultured for more than 40 generations and named as HOS-8603. The establishment of this cell line and its biological characteristics are briefly reported. 1 Materials and Methods 1.1 Materials On April 19, 1986, we obtained osteosarcoma resection specimens from Changhai Hospital of our university. The patients, male, aged 23,