基因转移方法为基因治疗的关键。目前 ,在动物及人体试验中应用最广的病毒载体是来源于致瘤型反转录病毒为代表的简单的反转录病毒载体 (RV) ,但该类载体不能有效地转染低分裂潜能细胞如终末分化的神经细胞、造血干细胞(HSC)、视网膜细胞、肝脏细胞、胰岛细胞等。慢病毒为反转 Gene transfer methods are the key to gene therapy. At present, the most widely used viral vectors in animal and human trials are simple retroviral vectors (RVs), typified by oncogenic retroviruses, but such vectors do not efficiently transfect low-division potential Cells such as terminally differentiated nerve cells, hematopoietic stem cells (HSCs), retinal cells, liver cells, islet cells and the like. Lentivirus is reverse