木薯是无性繁殖作物,每年都需设增殖苗圃以保存无性系种质。然而,病虫害有时会给某一优良种质造成毁灭性的威胁。分生组织的离体培养技术不仅为生产提供了无菌种苗,而且也为国际交换提供了种质材料。不过,这种组织培养法的缺点是必须定期换新鲜培养基,从而增加了污染的机会。1979年Bajai曾研究木薯分生组织超低温(-196 Cassava is a vegetatively propagated crop, and annual breeding nurseries are needed to preserve clonal germplasm. However, pests sometimes cause a devastating threat to a good germplasm. Mesenchymal ex-situ techniques not only provide sterile seedlings for production, but also provide germplasm for international exchange. However, the disadvantage of this tissue culture method is the need to change fresh media on a regular basis, thereby increasing the chances of contamination. In 1979 Bajai had studied cassava meristems cryogenic (-196