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目的探讨妊娠对小鼠肠黏膜免疫系统中T淋巴细胞活化的影响。方法取健康清洁级BALB/c近交系小鼠,雌性,6~8周龄,在孕14 d断颈处死6只小鼠制备淋巴细胞悬液;采用荧光素标记抗体技术分别检测未受刺激状态下和ConA刺激下派氏集合淋巴结(PP)、肠系膜淋巴结(MLN)、腹股沟淋巴结(ILN)来源的T淋巴细胞早、中、晚期活化标志物CD69、CD25、CD71的表达情况,与同期非孕鼠进行比较分析。结果孕鼠PP来源的T细胞CD69的表达率明显高于MLN和ILN来源的T细胞,差异均有统计学意义(P<0.01)。孕鼠PP来源的T细胞CD69的表达率明显高于非孕鼠,差异有统计学意义(P<0.05);CD25、CD71表达率在孕鼠和非孕鼠之间差异无统计学意义(P>0.05)。加入ConA刺激后不同来源的T细胞CD69、CD25、CD71表达率均明显高于未受刺激状态,差异有统计学意义(P<0.01)。加入ConA培养6h后,孕素PP来源的CD69表达率明显高于与非孕鼠,差异有统计学意义(P<0.01)。培养24h后,孕鼠和PP来源的CD25表达率与非孕鼠之间差异无统计学意义(P>0.05);孕鼠PP来源的CD25的表达率明显高于MLN、ILN来源的CD25,差异有统计学意义(P<0.01)。培养48h后,孕鼠PP来源的CD71表达率与非孕鼠之间差异无统计学意义(P>0.05)。结论妊娠过程可提升PP来源的T淋巴细胞CD69基础活化率,对MLN、ILN来源T淋巴细胞的影响仍需要进一步研究。
Objective To investigate the effect of pregnancy on the activation of T lymphocytes in the intestinal mucosal immune system in mice. Methods BALB / c inbred mice of healthy and clean grade, female, aged 6 to 8 weeks old, were sacrificed at the 14th day of gestation. Six mice were sacrificed to prepare lymphocyte suspension. Fluorescein-labeled antibody was used to detect unstimulated CD69, CD25 and CD71 in early, middle and late stages of T lymphocyte derived from Peyer’s patches (PP), mesenteric lymph nodes (MLN) and inguinal lymph nodes (ILN) under the stimulation of ConA, Pregnant rats for comparative analysis. Results The expression of CD69 in T cells derived from PP in pregnant rats was significantly higher than that in T cells derived from MLN and ILN (P <0.01). The positive rates of CD69 in T cells derived from PP in pregnant rats were significantly higher than those in non-pregnant rats (P <0.05). There was no significant difference in the expression rates of CD25 and CD71 between pregnant and non- > 0.05). The expression rates of CD69, CD25 and CD71 in T cells from different sources after adding ConA were significantly higher than those without stimulation (P <0.01). After adding ConA for 6h, the expression of progesterone-derived CD69 was significantly higher than that of non-pregnant mice (P <0.01). After culturing for 24 h, there was no significant difference in the expression rate of CD25 between pregnant rats and PP sources (P> 0.05). The expression of CD25 in PP-derived pregnant rats was significantly higher than that in MLN and ILN-derived CD25 There was statistical significance (P <0.01). After cultured for 48h, there was no significant difference in the expression of CD71 between PP pregnant mice and non-pregnant mice (P> 0.05). Conclusion Pregnancy can improve basal activation of CD69 on T lymphocytes, and further study on the effects of MLN and ILN on T lymphocytes.