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按Sawyer图谱在家兔双侧杏仁核(座标P_(2·0),L_(6·0),H_(15·0),A_1,L_(6·0),H_(13·0))埋植外径为0.7毫米的不锈钢套管。一周后实验,用热幅射一甩头法测痛,核内注射药物:氯化钙2微克/微升/侧、氯化镁1.7微克/微升/侧、环已二胺四乙酸(CDTA)6.25微克/微升/侧,异搏定(Verapamil)10微克/4微升/侧。除异搏定(16分钟)外、其余各药液均在8分钟内注射完毕。注射开始后10分钟静注吗啡,或用57—6D型电针仪发出的疏密式脉冲波(2～15赫,1伏)电针10分钟。每隔10分钟测痛一次,连续五次,将所测值与基础痛阈相比较,计算变化％。以+200％为最高值。
According to the Sawyer map, the expressions of Bcl-2 and Bax were detected in the bilateral amygdala (P 2,0 0, L 6,0 0, H 15,0, A 1, L 6,0, H 13,0) A 0.7 mm OD stainless steel sleeve is embedded. One week after the experiment, a heat stroke was used to measure the pain with a twitch head. The intracranial injection of drugs: calcium chloride 2 micrograms / microliter / side, magnesium chloride 1.7 micrograms / microliter / side, cyclohexane diamine tetraacetic acid (CDTA) 6.25 Μg / μl / side, Verapamil 10 μg / 4 μl / side. In addition to verapamil (16 minutes), the rest of the liquid injection were completed within 8 minutes. Morphine was intravenously administered 10 minutes after the start of the injection or a sparse pulse (2-15 Hz, 1 volt) electro-acupuncture with a 57-6D electroacupuncture instrument for 10 minutes. Pain measured every 10 minutes, five times in a row, the measured value and the basis of pain threshold compared to calculate the% change. The highest value of + 200%.