【摘 要】
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The myeloblastosis oncogenes (MYB) are important transcription factors that facilitate induction of variously develop-mental and stress responsive genes. They are hence, emerging as key players in improving stress tolerance of plants in response to severa
【机 构】
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College of Life Sciences,Northeast Agricultural University,Harbin 150030,China
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The myeloblastosis oncogenes (MYB) are important transcription factors that facilitate induction of variously develop-mental and stress responsive genes. They are hence, emerging as key players in improving stress tolerance of plants in response to several abiotic stresses. It was predicted that DfMYB2 contained an open reading frame (ORF) of 1621 bp coding 377 amino acid residues with molecular weight of 41.5 ku. It had 50 potential phosphorylation sites, 44 potential N-glycosylation sites and one trans-membrane domain. In neighbor-joining (NJ) phylogenetic tree, DfMYB2 was close to the branch of angiosperms. The subcellular localization of DfMYB2 was in nucleus. The expressions of DfMYB2 increased gradually in the filament, prothalli and sporophyte. The results showed that DfMYB2 played a more and more important role in its growth and development. And the expression levels in rhizomes were significantly higher than those in roots and leaves. After abscisic acid (ABA) and PEG600 treatment, DfMYB2 showed a downward trend, followed by an upward trend. The expression of DfMYB2 was inhibited in a short time under drought stress, the strong induction of DfMYB2 expression by ABA indicated that it was possibly involved in abiotic stress responses in an ABA-dependent manner.
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