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为选育出高功能成分含量的大麦新品种,以紫光芒裸二棱×Schooner构建的包含193个株系的RIL群体为材料,在玉溪和白邑2个生态点种植,对总黄酮、生物碱、γ-氨基丁酸和抗性淀粉含量进行测定及遗传分析。结果表明,不同生态条件下,大麦RIL群体籽粒4个被测功能成分的含量差异较大,群体间呈现广泛的遗传变异;总黄酮、生物碱及抗性淀粉含量均呈正态分布,表现为连续变异,由多基因控制;γ-氨基丁酸含量呈偏态分布,受主效基因控制。生态环境对大麦RIL群体籽粒4个被测功能成分含量的影响较大,低海拔高温气候有利于该群体总黄酮、γ-氨基丁酸及抗性淀粉含量的提升,高海拔冷寒气候有助于生物碱含量的增加。
In order to breed new varieties of barley with high content of functional components, 193 RIL populations were constructed from two populations of Dianthus chinensis × Schooner and were planted in two ecological sites of Yuxi and Baiyi. The total flavonoids, Alkali, γ-aminobutyric acid and resistant starch content determination and genetic analysis. The results showed that under different ecological conditions, the contents of four tested functional components of grain in RIL population of barley varied greatly, and the genetic variations among populations showed a wide range of genetic variation. The content of total flavonoids, alkaloids and resistant starch showed normal distribution Continuous variation, controlled by multiple genes; γ-aminobutyric acid content was skewed distribution, dominated by major genes. The ecological environment had a great influence on the content of four tested functional components in the barley RIL population. The low-altitude and high-temperature climate was beneficial to the increase of total flavonoids, γ-aminobutyric acid and resistant starch in this population, Increase in alkaloid content.