论文部分内容阅读
利用转基因技术创造苜蓿新种质已成为牧草新技术育种的重要组成部分。为了有效地从苜蓿转基因植株及其后代中选育出优良品种,深入研究转基因苜蓿的植物学性状及其产量十分重要。以通过农杆菌介导技术获得的T0代转BADH基因苜蓿为试材,利用分子生物学方法对其自交株系的世代群体连续进行抗盐性鉴定筛选和系统选育,首次获得了具有抗盐碱能力的转基因苜蓿稳定株系。同时,通过品种比较实验、区域实验和生产实验,表明在不同盐碱地条件下,转BADH基因的苜蓿植株产草量明显高于对照(未转基因的中苜1号),生产实验的干草增产率介于13.11%–24.98%之间。上述结果表明,外源目的基因主要特性的遗传稳定,进而从实践上验证了转BADH基因工程操作的实用性。
The use of transgenic technology to create a new alfalfa germplasm has become an important part of the new breeding technology of forage. In order to effectively select elite varieties from alfalfa transgenic plants and their offspring, it is very important to further study the botanical traits and yield of transgenic alfalfa. The T0 transgenic BADH gene alfalfa obtained by Agrobacterium tumefaciens-mediated technique was used as experimental material, and the progeny population of its inbred line was identified by molecular biology method for salt resistance screening and system selection for the first time. Saline - Alkalinity Transgenic Alfalfa Stable Lines. At the same time, through the variety comparison experiment, regional experiment and production experiment, it showed that under different saline-alkali conditions, the yield of alfalfa plants with BADH gene was significantly higher than that of the control (non-genetically modified Zhonglu No.1) Between 13.11% -24.98%. The above results show that the major traits of the exogenous gene are genetically stable, which in turn proves the practicability of transgenic BADH gene engineering.