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目的 研究TPMT rs12201199 A ＞ T和COMT rs9332377 C ＞ T基因多态性与顺铂所致成年人耳毒性的关系.方法 用PCR - Sanger测序法对37名接受顺铂治疗的肿瘤患者进行TPMT rs12201199 A ＞ T和COMT rs9332377 C ＞ T基因多态性分析,并跟踪记录患者在使用顺铂化疗前后听力变化情况.结果 37例肿瘤患者中COMT rs9332377 C ＞ T基因分型没有筛选出突变型,TPMT rs12201199 A ＞ T基因分型筛选出2例突变杂合子(AT基因型),35例野生纯合子(AA基因型);37例患者中有5例患者化疗前后出现一级以上听力损伤,AA和AT基因型患者发生听力损伤的频率没有显著性差异(P ＞ 0.05).结论 TPMT rs12201199 A ＞ T和COMT rs9332377 C ＞ T基因多态性与顺铂所致成年人耳毒性之间没有关系.“,”Objective To analyze the association of TPMT rs12201199 A ＞ T and COMT rs9332377 C ＞ T polymorphisms with cisplatin - induced ototoxicity in adults. Methods The polymorphisms of TPMT rs12201199 A ＞ T and COMT rs9332377 C ＞ T were detected by PCR - Sanger sequencing method in 37 cisplatin - treated patients and the change of hearing before and after cisplatin chemotherapy was followed up. Results The mutation genotype of COMT rs9332377 C ＞ T was not screened out in 37 cases of cancer patients. 2 mutation heterozygotes (AT genotype) and 35wild homozygotes were detected of TPMT rs12201199 A ＞ T genotyping. 5 patients had more than one grade of hearing impairment before and after chemotherapy in 37 patients, and the frequency of hearing loss in patients with AA and AT genotype had no significant difference (P ＞ 0.05). Conclusion TPMT rs12201199 A ＞ T and COMT rs9332377 C ＞ T genetic polymorphisms are not associated with cisplatin - induced ototoxicity in adults.