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用60Co-γ射线直接辐照龙特甫B干种子,诱发获得了多种类型叶色突变体。M2 代按苗计,白化、黄化和条纹三种叶色突变体的频率依次为0.347% ,0.041% 和0.031% 。对M2 代黄化和条纹两类突变群体的生长发育动态研究发现,其平均叶片数和分蘖数与对照有较大的差异。但有一些黄化和条纹突变体在三叶期后即可转为绿色,其叶片数和分蘖数与对照相近。在用这些叶色突变体与龙特甫A 杂交、回交后的M 4BC1 群体中,分离出正常绿色和带有叶色标记的两类不育株,二者之比接近1∶1。根据苗期和成株期的叶色特征,这些突变系分为 6 种类型。对M 4 突变系的株高、单株穗数、每穗总粒数、每穗实粒数和结实率及相应的 M4BC1 植株的株高和单株穗数进行了考察,发现转绿型突变系及其相应的M4BC1 带叶色标记植株的农艺性状与相应的对照相仿。
Irradiation of Longtefu B seeds with 60Co-γ ray directly induced many types of leaf color mutants. According to the seedling number of M2 generation, the frequency of three leaf color mutants of albino, yellow and stripe were 0.347%, 0.041% and 0.031% respectively. The M2 generation of yellowing and stripe two types of mutant population growth and development dynamics study found that the average number of leaves and tillers and the control there is a big difference. However, some yellowing and stripe mutants turned green after the three-leaf stage, with similar numbers of leaves and tillers. In the M 4BC1 population, which had been crossed with Longtefu A by these leaf color mutants and backcrossed, two types of sterile plants, normal green and leaf color, were isolated, the ratio of the two being close to 1: 1. According to the leaf color characteristics at seedling and adult stage, these mutations are divided into six types. The plant height, number of panicles per panicle, total number of panicles per panicle, number of solid grains per panicle, seed setting rate and corresponding plant height and panicle number of M4BC1 plants in M 4 mutant line were investigated. The agronomic traits of the M4BC1 plants with leaf color markers were similar to the corresponding controls.