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下丘脑通过分泌神经激素控制垂体前叶功能,继而调节体内肾上腺、甲状腺及性腺等内分泌器官的活动,唯有胰岛例外,它的活动似乎不受下丘脑的直接调节控制;但最近美国得克萨斯大学生理实验室Moltz等报道,他们从大鼠的下丘脑提纯出一种物质,可以刺激胰岛释放胰高血糖素,作者将其命名为胰高血糖素释放因子(glucagon releasing factor,简称GLRF)。作者将一万只大鼠的下丘脑正中隆起部分收集在一起(干重164g),先用盐酸、丙酮及石油醚粗提取,然后在G-25 Sephadex上做柱层析,对洗脱液各部分进行生物活性鉴定,观察其对离体胰腺和在体灌流胰腺释放胰岛素和胰高血糖素的刺激作用。实验结果表明,在提取液中存在着某种能特异性地刺激胰高血糖素释放的物质,但并不刺激胰岛素的释放。将具有生物活性的这部分洗脱液冰冻干燥,进一步用羧甲基纤
Hypothalamic secretion of neurohormones by controlling the anterior pituitary function, and then regulate the body’s adrenal gland, thyroid and gonadal and other endocrine organs activity, with the exception of the islet, its activity does not seem to be directly regulated by the hypothalamus; but recently the University of Texas physiology Laboratory Moltz et al. Reported that they purified a substance from the hypothalamus of rats that stimulated islets to release glucagon, which the authors named as glucagon releasing factor (GLRF). The author collected the uplift of the hypothalamus of 10,000 rats (dry weight 164g), first with hydrochloric acid, acetone and petroleum ether, then column chromatography on G-25 Sephadex, the eluent Part of biological activity identification, observed in vitro pancreatic and in vivo perfused pancreas release of insulin and glucagon stimulation. The experimental results show that there is some substance that can specifically stimulate glucagon release in the extract, but it does not stimulate the release of insulin. The biologically active fractions were lyophilized and further treated with carboxymethylcellulose