论文部分内容阅读
背景:溃疡性结肠炎(UC)是由环境、感染、免疫、遗传等多因素相互作用所致的肠道慢性炎症,其中遗传易感性可能在UC的发病中起重要作用。目的:了解中国汉族UC患者环氧合酶(COX)-23’非翻译区8473T/C位点基因多态性及其与UC发病的可能相关性。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法对291例UC患者和286名健康对照者的COX-23’非翻译区8473T/C位点的单核苷酸多态性进行检测。结果:与健康对照组比较,UC组COX-28473T/C位点TT基因型频率较低(58.8%对64.3%),而TC、CC基因型频率较高(36.8%对33.9%、4.5%对1.7%),但差异均无统计学意义(P>0.05)。两组受检者T等位基因频率均较高,两组间C等位基因频率差异无统计学意义(P=0.092)。结论:COX-23’非翻译区8473T/C位点基因多态性与中国汉族UC患者的遗传易感性无相关性。
BACKGROUND: Ulcerative colitis (UC) is a chronic inflammatory bowel disease caused by multiple factors such as environment, infection, immunity and heredity. Genetic susceptibility may play an important role in the pathogenesis of UC. Objective: To investigate the gene polymorphism of 8473T / C of cyclooxygenase (COX) -23 ’untranslated region and its possible relationship with UC in Chinese Han patients. Methods: The single nucleotide of 8473T / C in COX-23 ’untranslated region of 291 UC patients and 286 healthy controls were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) Polymorphism was tested. Results: Compared with healthy controls, the frequency of TT genotypes in COX-28473T / C locus was lower in UC patients (58.8% vs 64.3%), while frequencies of TC and CC genotypes were higher (36.8% vs 33.9%, 4.5% vs 1.7%), but the differences were not statistically significant (P> 0.05). The frequency of T allele was higher in both groups, and there was no significant difference in the frequency of C allele between the two groups (P = 0.092). Conclusion: There is no correlation between gene polymorphism at 8473T / C in COX-23 ’untranslated region and genetic predisposition of UC patients in Chinese Han population.