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目的:研究清开灵对东莨菪碱致小鼠空间学习记忆障碍的作用及脑内一氧化氮(NO)和一氧化氮合酶(NOS)的影响。方法:东莨菪碱造模的雄性KM小鼠随机分为6组,正常对照组、模型对照组、清开灵20g/kg组、清开灵40g/kg、清开灵80 g/kg组和阳性对照药尼莫地平0.3g/kg组。给予等容积药物或蒸馏水,每日灌胃一次,连续30天。于末次给药后30min,除对照组腹腔注射生理盐水外,其它各组注射东莨菪碱2mg/kg,20min后进行Morris水迷宫测试,测试5天后处死,取其脑组织进行NO和NOS测定。结果:清开灵可明显改善东莨菪碱所致小鼠的空间学习记忆障碍,并可以降低小鼠脑内NO含量和NOS活性。结论:清开灵能明显改善东莨菪碱所致小鼠空间学习记忆障碍,并降低其脑内NO含量及NOS活性。
Objective: To investigate the effect of Qingkailing on scopolamine-induced spatial learning and memory impairment and the effects of nitric oxide (NO) and nitric oxide synthase (NOS) in brain of mice. Methods: Male KM mice scopolamine model were randomly divided into 6 groups: normal control group, model control group, Qingkailing 20g / kg group, Qingkailing 40g / kg, Qingkailing 80g / kg group and positive control Nimodipine 0.3g / kg group. Give equal volume of medicine or distilled water, gavage once daily for 30 consecutive days. At 30 minutes after the last administration, all rats in the control group were injected scopolamine 2 mg / kg intraperitoneally except for the control group. Morris water maze test was performed 20 minutes after the last administration. After 5 days of the test, the rats were sacrificed and their brain tissues were taken for determination of NO and NOS. Results: Qingkailing can significantly improve scopolamine-induced spatial learning and memory impairment, and can reduce the content of brain NO and NOS activity in mice. Conclusion: Qingkailing can significantly improve scopolamine-induced spatial learning and memory impairment, and reduce the brain NO content and NOS activity.