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给予大鼠左侧胸 9脊神经背侧皮支或其外周断端较强电刺激后 ,用原位杂交方法观察相邻节段背根节 PPT m RNA表达的变化。结果表明 ,电刺激完整神经后 2 4h,同侧胸 8、胸 9和胸 10背根节 PPT m RNA表达的阳性细胞数明显高于对照组 ( P<0 .0 5 )。电刺激断离中枢的神经外周端后 2 4h,同侧胸 8和胸 10背根节 PPT m RNA表达的阳性细胞数明显高于对照组 ( P<0 .0 5 )。表明较强的逆行电刺激 ,可以在外周通过跨节段传递信息 ,引起相邻节段背根节的 PPT m RNA表达的增强。提示由 PPT生成的 SP等生物活性物质在跨节段信息传递的过程中起着重要作用
The left dorsal horn of the dorsal horn of the dorsal root ganglia of 9 rats was stimulated by electrical stimulation. The in situ hybridization was used to observe the expression of PPT m RNA in adjacent dorsal root ganglia. The results showed that the number of PPT m RNA positive cells in ipsilateral chest 8, chest 9 and thoracic dorsal root ganglion was significantly higher than that in control group (P <0.05) at 24 hours after electrical stimulation of intact nerve. The number of PPT m RNA positive cells in the ipsilateral chest 8 and thoracic dorsal root ganglion was significantly higher than that in the control group (P <0.05) at 24 h after electrical stimulation of the peripheral nerve end. Suggesting that strong retrograde electrical stimulation can transmit information across the segments in the periphery, resulting in increased expression of PPT m RNA in adjacent segments of the dorsal root ganglia. It is suggested that the bioactive substances such as SP produced by PPT play an important role in the cross-sectional information transmission