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研究了丝孢酵母（Ｔｒｉｃｈｏｓｐｏｒｏｎ ｓｐ）的不同处理和接种时间对“富士”苹果灰霉病和青霉病的抑制效果。结果表明，当接种灰霉菌和青霉菌孢子浓度分别为１×１０５个／ｍｌ和５×１０４个／ｍｌ时，在２５℃下．１×１０８ＣＦＵ／ｍｌ的酵母悬浮液完全抑制这两种病害的发生；在 １℃下冷藏 ３０ｄ后．灰霉病和青霉病的发病率分别为 １３％和 ０。接种酵母菌的滤液对病害没有抑制作用。Ｔｒｉｃｈｏｓｐｏｒｏｎ ｓｐ能在苹果伤口迅速繁殖，以 ２５℃ 下最初的 ４８ｈ内和 １℃下的最初 ５ｄ内增长最快，分别增加了５０和２０倍以上，以后基本维持在此水平。酵母菌和病菌孢子的接种时间与生物防治效果有关，先接种拮抗菌的抑菌效果显著地好于同时或后于病菌接种的效果。
The inhibitory effects of different treatments and inoculation times of Trichosporon sp on gray mold and blue mold of “Fuji” apple were studied. The results showed that when the spore concentrations of Botrytis cinerea and Penicillium sp. Were 1 × 10 5 / ml and 5 × 10 4 / ml, respectively, at 25 ℃. The yeast suspension at 1 x 108 CFU / ml completely inhibited the occurrence of both diseases; after cooling at 1 ° C for 30 days. The incidence of gray mold and blue mold was 13% and 0 respectively. Inoculation of yeast filtrate did not inhibit the disease. Trichosporon sp could rapidly multiply in apple wounds, with the fastest growth in the first 48 hours at 25 ° C and the first 5 days at 1 ° C, with an increase of more than 50 and 20 times, respectively, after which Trichosporon sp basically maintained at this level. The inoculation time of yeasts and bacteria spores was related to the biological control effect. The antibacterial effect of the first inoculation of antagonistic bacteria was significantly better than that of the same or later inoculation.